#LIFE_SCIENCE_CONCEPTS
SDS and Native PAGE Techniques:-
In native PAGE, proteins are separated according to the net charge, size, and shape of their native structure. Electrophoretic migration occurs because most proteins carry a net negative charge in alkaline running buffers. The higher the negative charge density (more charges per molecule mass), the faster a protein will tend to migrate. At the same time, the frictional force of the gel matrix creates a sieving effect, regulating the movement of proteins according to their size and three-dimensional shape. Small proteins face only a small frictional force, while larger proteins face a larger frictional force. Thus, native PAGE separates proteins based upon both their charge, mass and structure.
Because no denaturants are used in native PAGE, subunit interactions within a multimeric protein are generally retained and information can be gained about the quaternary structure. In addition, some proteins retain their enzymatic activity (function) following separation by native PAGE. Thus, this technique may be used for preparation of purified, active proteins.
SDS-PAGE separates proteins primarily by mass because the ionic detergent SDS denatures and binds to proteins to make them uniformly negatively charged. Thus, when a current is applied, all SDS-bound proteins in a sample will migrate through the gel toward the positively charged electrode.
The major difference between native PAGE and SDS-PAGE is that in native PAGE, the protein migration rate is dependent on both the mass and structure, whereas in SDS-PAGE, the migration rate is determined only by protein's mass. In native PAGE, protein samples are prepared in a non-denaturing and non-reducing buffer.
https://www.facebook.com/lifescience.concept/
https://www.facebook.com/Lifescienceclass
https://t.me/joinchat/bTKh8oX34fRjMmY1
https://t.me/LIFESCIENCE_CONCEPTS
https://www.instagram.com/invites/contact/?i=1sez59bii4nkj&utm_content=36rla7t
csirlifescienceclasses@gmail.com
#SDS_and_Native_PAGE_Techniques
#CSIR_JUNE_2019_SEC_B_MCQ
CSIR_LIFE_SCIENCE
CSIR _JRF
CSIR_UGC_NET
GATE
GATE_BT
GATE_XL
ICAR_AICE_JRF
ICAR_ASRB_NET
ICMR_JRF
DBT_JRF
ACTREC_JRF
UGC_NET
TIFR
JNCASR
IISER
CDRI
CCMB
NCCS
NII
NBRC
BITS_PILANI
DRDO
BINC
IISC
LIFE_SCIENCE
IIT_JAM
M.Sc_ENTRANCE
JNU
BHU
DU
#LIFE_SCIENCE_CONCEPTS
#lifescienceconcepts
#life_science_concepts
#lifescience_concepts
#life_scienceconcepts
#Life_Science_Concepts
#Lifescienceconcepts
#Lifescience_concepts
#life_scienceconcepts
0 Comments